SpotOn is a robust algorithm developed to identify and classify the interfacial residues as Hot-Spots (HS) and Null-Spots (NS) with a final accuracy of 0.95 and a sensitivity of 0.95 on an independent test set.

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This work is co-funded by the Horizon 2020 projects EOSC-hub (grant number 777536) and BioExcel (grant numbers 823830 and 675728). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no [MEMBRANEPROT 659826].